Ecl Western Blot Detection System

Ecl substrates for high sensitivity western blot detection chemiluminescent assays depend on the emission of light as a product of a chemical reaction and are commonly used for the detection of proteins on western blots.

Ecl western blot detection system.

General description ecl western blotting system is the world s most widely used and referenced chemiluminescent immunodetection system. Ecl western blotting system uses horseradish peroxidase hrp conjugated anti mouse and anti rabbit antibodies for luminol based detection of western blots. Expression and purification of gst tagged proteins can be monitored by western blot analysis using amersham ecl amersham ecl prime or amersham ecl select detection systems to enhance sensitivity. It enables the detection of picogram amounts of antigen and allows for easy detection of hrp using photographic or other imaging methods.

Detection of gst tagged proteins with western blot. Anti mouse hrp conjugate 100 µl anti rabbit hrp conjugate 100 µl ecl detection reagents 1 and 2 62 5 ml each blocking agent 5 g. Ecl western blotting system is the world s most widely used and referenced chemiluminescent immunodetection system. Pierce ecl western blotting substrate provides reliability and performance equivalent to other standard ecl substrates for detection of hrp enzyme activity.

Improving the overall efficiency of electrophoresis systems these reagents. Blots probed with mouse or rabbit primary antibodies are incubated with hrp conjugated. Contents include the following. Ecl western blotting detection reagents in combination with hybond ecl membranes form a complete solution for western blot applications.